湖南师范大学自然科学学报

2020, v.43;No.183(04) 43-49

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藜麦WOX转录因子家族的鉴定及表达分析
Identification and Expression Analysis of WOX Transcription Factor Family of Chenopodium quinoa

朱满喜;杨雅舒;杨小兰;张芳;杨利艳;王创云;
ZHU Man-xi;YANG Ya-shu;YANG Xiao-lan;ZHANG Fang;YANG Li-yan;WANG Chuang-yun;College of Life Science, Shanxi Normal University;Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences;

摘要(Abstract):

为揭示藜麦WOX基因家族的功能,为藜麦耐低氮品种的培育提供参考,利用生物信息学方法,对藜麦WOX基因进行全基因组鉴定,并对其理化性质、系统发育、基因结构、保守结构域、保守基序、组织表达和响应氮胁迫的表达进行分析。研究结果表明:藜麦中共有13个WOX转录因子,蛋白长度159~676 aa,相对分子质量18 510~76 730,等电点5.27~9.56;每个成员含有1~4个内含子及2~5个外显子, WOX蛋白都具有由34~60个氨基酸组成的Homeobox保守结构域。系统进化树显示,来源于拟南芥、水稻和藜麦的39个WOX基因分为3组,每一组都含有3个物种的家族成员,同一个物种的WOX基因具有更近的遗传距离。组织表达热图显示,藜麦WOX基因组织表达特异性明显,在茎中的表达量最高,其次是种子和叶,在幼苗中的表达量最低。AUR62031363和AUR62035466对氮胁迫的响应最为显著。
In order to reveal the function of the quinoa WOX gene family and provide references for the cultivation of quinoa varieties tolerant to low nitrogen, in this work, the whole genome of quinoa WOX genes was identified by bioinformatics method. Its physical and chemical properties, phylogeny, gene structure, conserved domain, conserved motif, tissue expression and expression in response to nitrogen stress were subsequently analyzed. Our results show that there are 13 WOX transcription factors in quinoa, with the protein length between 159~676 aa, molecular weight between 18 510~76 730, isoelectric point between 5.27~9.56, and each containing 1~4 introns and 2~5 exons. WOX proteins have a Homeobox conserved domain consisting of 34~60 amino acids. The result of the phylogenetic tree analysis showed that 39 WOX genes from Arabidopsis thaliana, rice, and quinoa could be grouped into 3 clusters, with each group containing WOX genes from above 3 species. WOX genes from the same species exhibited a closer genetic distance. Tissue expression analysis data with heat map showed that WOX gene expression was tissue specific, in which the highest gene expression presented in stems, then in seeds and leaves, and lowest in seedlings. AUR62031363 and AUR62035466 displayed significant responses to nitrogen stresses.

关键词(KeyWords): 藜麦;WOX转录因子;生物信息学;基因表达;氮胁迫
quinoa;WOX transcription factor;bioinformatics;gene expression;nitrogen stress

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基金项目(Foundation): 山西省应用基础研究计划项目(201901D111285);; 院士工作站专项资助项目(20190423)

作者(Author): 朱满喜;杨雅舒;杨小兰;张芳;杨利艳;王创云;
ZHU Man-xi;YANG Ya-shu;YANG Xiao-lan;ZHANG Fang;YANG Li-yan;WANG Chuang-yun;College of Life Science, Shanxi Normal University;Institute of Crop Sciences, Shanxi Academy of Agricultural Sciences;

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